| 质粒名称: | pcDNA3.1 MYC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 176045 |
| 存储实验室: | Linda Penn |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 6000bp |
| 完整质粒大小: | 7325bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Myc、c-Myc |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1320bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed) |
| 3’克隆位点: | EcoRI (not destroyed) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | Myc_pSeq3R |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1 MYC was a gift from Linda Penn (Addgene plasmid # 176045 ; http://n2t.net/addgene:176045 ; RRID:Addgene_176045) |
| 参考文献部分: | MYC Interacts with the G9a Histone Methyltransferase to Drive Transcriptional Repression and Tumorigenesis. Tu WB, Shiah YJ, Lourenco C, Mullen PJ, Dingar D, Redel C, Tamachi A, Ba-Alawi W, Aman A, Al-Awar R, Cescon DW, Haibe-Kains B, Arrowsmith CH, Raught B, Boutros PC, Penn LZ. Cancer Cell. 2018 Oct 8;34(4):579-595.e8. doi: 10.1016/j.ccell.2018.09.001. 10.1016/j.ccell.2018.09.001 PubMed 30300580 |
买家导航
pcDNA3.1 MYC载体质粒基本信息
pcDNA3.1 MYC质粒图谱载体图谱和pcDNA3.1 MYC载体序列质粒序列多克隆位点信息
pcDNA3.1 MYC质粒载体简介
pcDNA3.1 MYC质粒序列载体序列
LOCUS pcDNA3.1 MYC 7303 bp DNA circular SYN 25-AUG-2024
DEFINITION Expresses Myc in Mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7303)
AUTHORS Tu WB, Shiah YJ, Lourenco C, Mullen PJ, Dingar D, Redel C, Tamachi
A, Ba-Alawi W, Aman A, Al-Awar R, Cescon DW, Haibe-Kains B,
Arrowsmith CH, Raught B, Boutros PC, Penn LZ
TITLE MYC Interacts with the G9a Histone Methyltransferase to Drive
Transcriptional Repression and Tumorigenesis.
JOURNAL Cancer Cell. 2018 Oct 8;34(4):579-595.e8. doi:
10.1016/j.ccell.2018.09.001.
PUBMED 30300580
REFERENCE 2 (bases 1 to 7303)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.ccell.2018.09"; journalName: "Cancer Cell"; date:
"2018-10-8- 8"; volume: "34"; issue: "4"; pages: "579-595.e8"
FEATURES Location/Qualifiers
source 1..7303
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 55..74
/label=T7
/note="T7 promoter, forward primer"
promoter 55..73
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 373..1692
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
promoter complement(2048..2066)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2049..2066)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2086..2103)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2092..2316
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2362..2790
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2449..2468)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2659..2680
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2799..2819)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2804..3133
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2984..3119
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3046..3065
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3200..3994
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3254..3273)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3864..3883
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4168..4289
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4205..4224)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4259..4278
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4338..4354)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4338..4354)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4351..4373)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4362..4378
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4386..4416)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4431..4452
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4569..4586)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4740..5328)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4820..4839)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5499..6359)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6122..6141
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6360..6464)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6539..6558)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 6730..7109
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter join(7110..7303,1..10)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 7264..7284
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
ORIGIN
1 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
61 gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc
121 agtgtgctgg aattcggccc cccccccctt gccgcatcca cgaaactttg cccatagcag
181 cgggcgggca ctttgcactg gaacttacaa cacccgagca aggacgcgac tctcccgacg
241 cggggaggct attctgccca tttggggaca cttccccgcc gctgccagga cccgcttctc
301 tgaaaggctc tccttgcagc tgcttagacg ctggattttt ttcgggtagt ggaaaaccag
361 cctcccgcga cgatgcccct caacgttagc ttcaccaaca ggaactatga cctcgactac
421 gactcggtgc agccgtattt ctactgcgac gaggaggaga acttctacca gcagcagcag
481 cagagcgagc tgcagccccc ggcgcccagc gaggatatct ggaagaaatt cgagctgctg
541 cccaccccgc ccctgtcccc tagccgccgc tccgggctct gctcgccctc ctacgttgcg
601 gtcacaccct tctcccttcg gggagacaac gacggcggtg gcgggagctt ctccacggcc
661 gaccagctgg agatggtgac cgagctgctg ggaggagaca tggtgaacca gagtttcatc
721 tgcgacccgg acgacgagac cttcatcaaa aacatcatca tccaggactg tatgtggagc
781 ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg cctcctacca ggctgcgcgc
841 aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg tctgctccac ctccagcttg
901 tacctgcagg atctgagcgc cgccgcctca gagtgcatcg acccctcggt ggtcttcccc
961 taccctctca acgacagcag ctcgcccaag tcctgcgcct cgcaagactc cagcgccttc
1021 tctccgtcct cggattctct gctctcctcg acggagtcct ccccgcaggg cagccccgag
1081 cccctggtgc tccatgagga gacaccgccc accaccagca gcgactctga ggaggaacaa
1141 gaagatgagg aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc tggcaaaagg
1201 tcagagtctg gatcaccttc tgctggaggc cacagcaaac ctcctcacag cccactggtc
1261 ctcaagaggt gccacgtctc cacacatcag cacaactacg cagcgcctcc ctccactcgg
1321 aaggactatc ctgctgccaa gagggtcaag ttggacagtg tcagagtcct gagacagatc
1381 agcaacaacc gaaaatgcac cagccccagg tcctcggaca ccgaggagaa tgtcaagagg
1441 cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc
1501 ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt agttatcctt
1561 aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct catttctgaa
1621 gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca gctacggaac
1681 tcttgtgcgt aaggaaaagt aaggaaaatg attccttcta acagaaatgt cctgagcaat
1741 cacctatgaa cttgtttcaa atgcatgatc aaatgcaacc tcacaacctt ggctgagtct
1801 tgagactgaa agatttagcc ataatgtaaa ctgcctcaaa ttggactttg ggcataaaag
1861 aactttttta tgcttaccat cttttttttt tctttaacag atttgtattt aagaattgtt
1921 tttaaaaaat tttaagattt acacaatgtt tctctgtaaa tattgccatt aaatgtaaat
1981 aacttccgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
2041 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
2101 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
2161 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
2221 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
2281 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
2341 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
2401 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
2461 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
2521 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
2581 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
2641 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
2701 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
2761 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
2821 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2881 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2941 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
3001 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
3061 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
3121 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
3181 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
3241 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
3301 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
3361 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
3421 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
3481 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
3541 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
3601 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
3661 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
3721 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
3781 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
3841 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
3901 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
3961 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
4021 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
4081 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
4141 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
4201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
4261 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
4321 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
4381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
4441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
4501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
4561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
5041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
5101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
5161 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
5221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
5281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
5341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
6001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
6061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
6121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
6181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
6241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
6301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6481 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
6541 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
6601 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
6661 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
6721 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
6781 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
6841 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
6901 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
6961 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
7021 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
7081 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
7141 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
7201 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
7261 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tat
//
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